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Immunohistochemistry and special stain of UBFT ‐TD bone marrow biopsy. ( A ) Naphthol‐Chloracetatesterase stain shows a reduced granulopoeisis (red). ( B ) Erythropoiesis is atypically located, with expanded erythroid islets and an increased presence of immature cell forms (E‐cadherin immunohistochemistry). ( C ) Megakaryopoiesis is hyperplastic with small groups of hypolobulated cell forms and micromegakaryocytes (CD42b immunohistochemistry). Myeloblasts are negative for CD34 ( D ; CD34 immunohistochemistry) and positive for CD33 ( E ; CD33 immunohistochemistry). MPO‐positive myeloid precursors show prominent UBTF‐positive nucleoli ( F ; UBTF and MPO double‐immunofluorescence). Dispersed CD3‐positive T‐lymphocytes and CD20‐positive B‐lymphocytes are visible ( G ; CD3 immunohistochemistry and H ; CD20 immunohistochemistry). Small aggregates of <t>CD123‐positive</t> plasmocytoid dendritic cells are shown ( I ; CD123 immunohistochemistry). EWOG ID number D1000. [Color figure can be viewed at wileyonlinelibrary.com ]
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Immunohistochemistry and special stain of UBFT ‐TD bone marrow biopsy. ( A ) Naphthol‐Chloracetatesterase stain shows a reduced granulopoeisis (red). ( B ) Erythropoiesis is atypically located, with expanded erythroid islets and an increased presence of immature cell forms (E‐cadherin immunohistochemistry). ( C ) Megakaryopoiesis is hyperplastic with small groups of hypolobulated cell forms and micromegakaryocytes (CD42b immunohistochemistry). Myeloblasts are negative for CD34 ( D ; CD34 immunohistochemistry) and positive for CD33 ( E ; CD33 immunohistochemistry). MPO‐positive myeloid precursors show prominent UBTF‐positive nucleoli ( F ; UBTF and MPO double‐immunofluorescence). Dispersed CD3‐positive T‐lymphocytes and CD20‐positive B‐lymphocytes are visible ( G ; CD3 immunohistochemistry and H ; CD20 immunohistochemistry). Small aggregates of <t>CD123‐positive</t> plasmocytoid dendritic cells are shown ( I ; CD123 immunohistochemistry). EWOG ID number D1000. [Color figure can be viewed at wileyonlinelibrary.com ]
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Immunohistochemistry and special stain of UBFT ‐TD bone marrow biopsy. ( A ) Naphthol‐Chloracetatesterase stain shows a reduced granulopoeisis (red). ( B ) Erythropoiesis is atypically located, with expanded erythroid islets and an increased presence of immature cell forms (E‐cadherin immunohistochemistry). ( C ) Megakaryopoiesis is hyperplastic with small groups of hypolobulated cell forms and micromegakaryocytes (CD42b immunohistochemistry). Myeloblasts are negative for CD34 ( D ; CD34 immunohistochemistry) and positive for CD33 ( E ; CD33 immunohistochemistry). MPO‐positive myeloid precursors show prominent UBTF‐positive nucleoli ( F ; UBTF and MPO double‐immunofluorescence). Dispersed CD3‐positive T‐lymphocytes and CD20‐positive B‐lymphocytes are visible ( G ; CD3 immunohistochemistry and H ; CD20 immunohistochemistry). Small aggregates of CD123‐positive plasmocytoid dendritic cells are shown ( I ; CD123 immunohistochemistry). EWOG ID number D1000. [Color figure can be viewed at wileyonlinelibrary.com ]

Journal: Histopathology

Article Title: Diagnostic features in paediatric MDS ‐ EB with UBTF ‐internal tandem duplication: defining a unique subgroup

doi: 10.1111/his.15378

Figure Lengend Snippet: Immunohistochemistry and special stain of UBFT ‐TD bone marrow biopsy. ( A ) Naphthol‐Chloracetatesterase stain shows a reduced granulopoeisis (red). ( B ) Erythropoiesis is atypically located, with expanded erythroid islets and an increased presence of immature cell forms (E‐cadherin immunohistochemistry). ( C ) Megakaryopoiesis is hyperplastic with small groups of hypolobulated cell forms and micromegakaryocytes (CD42b immunohistochemistry). Myeloblasts are negative for CD34 ( D ; CD34 immunohistochemistry) and positive for CD33 ( E ; CD33 immunohistochemistry). MPO‐positive myeloid precursors show prominent UBTF‐positive nucleoli ( F ; UBTF and MPO double‐immunofluorescence). Dispersed CD3‐positive T‐lymphocytes and CD20‐positive B‐lymphocytes are visible ( G ; CD3 immunohistochemistry and H ; CD20 immunohistochemistry). Small aggregates of CD123‐positive plasmocytoid dendritic cells are shown ( I ; CD123 immunohistochemistry). EWOG ID number D1000. [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Immunostaining was performed for CD42b (SP219; Abcam, Cambridge, UK), E‐cadherin (EP700y; Zytomed Systems, Berlin, Germany), MPO (Agilent, Santa Clara, CA, USA), TdT (SEN28; Leica, Deer Park, IL, USA), CD20 (L26; Agilent), CD3(SP7; Zytomed Systems), CD123 (AbcamK), CD34 (QBEnd/10; Cell Marque, Rocklin, CA, USA) using an autostainer (Benchmark Ultra; Roche Diagnostics, Germany) following the manufacturer's instructions.

Techniques: Immunohistochemistry, Staining, Immunofluorescence